Adipose Cell Differentiation in Explant-derived Cell Culture

06/05/2020
by   Daniel Cebo, et al.
0

Adipose tissue is derived from the embryonic mesenchyme and contains a stroma that can be easily isolated. Preliminary studies have recently identified a putative stem cell population within the adipose stromal compartment. This cell population can differentiate to the osteogenic, adipogenic, myogenic, and chondrogenic lineages. Enzymatic digestion, the commonly used method of adipose-derived stromal cells isolation, is time consuming and expensive, especially when applied to large volumes of tissue. Additionally, mechanical stress during isolation, use of bacterial-derived products and potential contamination with endotoxins and xenoantigens are other disadvantages of this method. In this study, the characteristics of the cells obtained by adipose tissue explant culture were studied. This technique can be used to reproducibly isolate mesenchymal stromal cells from fat tissue obtained by liposuction as well as surgical resection. Explant culture gave higher yield of cells than digestion method after primary culture. Therefore, explant culture can be used as an effective way to isolate adipose-derived stromal cells for tissue engineering and regenerative medicine, especially in cases of limited starting material.

READ FULL TEXT

page 1

page 2

page 3

research
06/05/2020

Characterization Of Bovine Adipose-Derived Stem Cells

Bovine adipose-derived stem cells were obtained from the subcutaneous ab...
research
07/18/2020

Automated Phenotyping via Cell Auto Training (CAT) on the Cell DIVE Platform

We present a method for automatic cell classification in tissue samples ...
research
05/30/2011

Alignment of Microtubule Imagery

This work discusses preliminary work aimed at simulating and visualizing...
research
11/21/2021

Public Opinion About Stem Cell Research and Human Cloning - The Bioethics of Stem Cell Research and Therapy

Discussion of the bioethics of human stem cell research has transitioned...
research
02/03/2018

Stochastic simulation of pattern formation in growing tissue: a multilevel approach

We take up the challenge of designing realistic computational models of ...
research
04/09/2021

What do cells regulate in soft tissues on short time scales?

Cells within living soft biological tissues seem to promote the maintena...
research
01/06/2023

A survey on Organoid Image Analysis Platforms

An in-vitro cell culture system is used for biological discoveries and h...

Please sign up or login with your details

Forgot password? Click here to reset